NAPPA Protein Array Facility

The NAPPA Protein Array Facility located within the Virginia G. Piper Biodesign Center for Personalized Diagnostics uses a novel protein microarray technology, called Nucleic Acid-Programmable Protein Array (NAPPA), which replaces the complex process of spotting purified proteins with the simple process of spotting plasmid DNA

NAPPA Facility Services:

  1. High-throughput plasmid DNA preparation
  2. Pre-set and custom full-length and protein domain arrays
  3. Array screening services

Applications:

  1. Serum screening to identify antibody immune responses for a variety of diseases including cancer, diabetes, autism and infectious diseases
  2. Detect post-translational modifications such as phosphorylation, AMPylation, or citrullination
  3. Identify protein-protein or protein-DNA interactions
  4. Quantify protein binding kinetics in high throughput by coupling NAPPA with surface plasmon resonance (SPRi)