NAPPA Protein Array Facility
The NAPPA Protein Array Facility located within the Virginia G. Piper Biodesign Center for Personalized Diagnostics uses a novel protein microarray technology, called Nucleic Acid-Programmable Protein Array (NAPPA), which replaces the complex process of spotting purified proteins with the simple process of spotting plasmid DNA
NAPPA Facility Services:
- High-throughput plasmid DNA preparation
- Pre-set and custom full-length and protein domain arrays
- Array screening services
Applications:
- Serum screening to identify antibody immune responses for a variety of diseases including cancer, diabetes, autism and infectious diseases
- Detect post-translational modifications such as phosphorylation, AMPylation, or citrullination
- Identify protein-protein or protein-DNA interactions
- Quantify protein binding kinetics in high throughput by coupling NAPPA with surface plasmon resonance (SPRi)