Shared Instrument Facility

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About

Contacts

Kyle Lewis
Shared Instrument Core
Facility Director
[email protected]

Location

Biodesign Shared Instrument Core
The Biodesign Institute
1001 S McAllister Ave | Tempe
Room A335A
Enter through 335 or 339

Overview of rules

  • All Shared Instrument Facilities are free of charge to Biodesign faculty and staff
  • All equipment is self-serve
  • All instruments are used on a first-come, first-serve basis
  • Access and training for all instruments is coordinated and supported by Kyle Lewis
  • Instrumental maintenance and calibration performed by Biodesign Facilities
  • Lab courtesy rules apply
  • ASU gas safety and instrument-specific training required for Jasco J-815 Spectropolariometer
  • Instrument-specific training required for Nanosight NS300

Overview of tools

Jasco J-815 Circular Dichroism Spectropolarimeter

Measures and quantifies differences in absorption of left and right circularly polarized light in chiral molecules and macromolecular structures.

  • Analyze secondary/tertiary structure or conformation of macromolecules
  • Observe how secondary structure of proteins changes with environmental conditions or on interaction with other molecules
  • Derive structural, kinetic and thermodynamic information
  • ASU gas handling and instrument specific training required
  • Researchers will provide their own cells / cuvettes; recommendations available based on application

Spectramax M5 plate reader (96/384 format)

Multimode photometrics for endpoint or kinetic measurements

  • Absorbance (UV – VIS)
  • Fluorescence
  • Glow luminescence
  • Time-resolved fluorescence
  • Fluorescent polarization

ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX Calibrated

Fluorescence based monitoring of PCR in real time to quantify nucleic acid transcripts.

  • Gene expression assays / Genotyping analysis
  • High resolution melt analysis
  • miRNA and iRNA quantification
  • Single cell PCR
  • TaqMan Array Card ready

Biorad C1000 Thermal Cycler

Two module tube strip (48 tubes per module) multi-use programmable thermal cycler

  • Endpoint PCR
  • Cloning reactions
  • Provided as an overflow instrument for labs needing extra thermal cycler space
  • Modules are independently programmable

GE ImageQuant LAS 4000

Basic CCD camera based blot / gel imaging

  • General purpose gel and blot Imaging
  • Chemiluminescence and fluorescence modes
  • RGB Epi-illumination
  • UV Trans-illumination
  • Software allows incremental image accumulation
  • NO ETHIDIUM BROMIDE GELS – “safe” stains only

NanoDrop 8000 Spectrophotometer

Simple, hand-pipetted pedestal based spectrophotometer

  • 8 channel model – 1uL required 2uL recommended
  • Rapid DNA/RNA/Protein quantification
  • Purity indicators A260/280 and A260/230 ratios
  • Preconfigured methods for BCA, Bradford, Pierce 660, and Lowry assays

NanoSight NS300 Particle Characterization Platform

NTA (Brownian motion tracking) based size characterization and quantitation of particles

  • 10 – 2000nm size range
  • 106 – 109 particles / mL
  • Viral vaccine and virus characterization
  • Phage assembly
  • Biomarker detection
  • Protein aggregation
  • Extracellular vesicle characterization

Hosted equipment

Hosted items are owned or supported by facilities but located within individual biodesign centers that have a complimentary research focus and large user base. All hosted items are available upon request and training completion, if applicable.

If you would like access to hosted equipment, to host a piece of equipment, or to offer an existing piece of equipment as a hosted item please contact Kyle Lewis.

  • BioRad GelDoc EZ gel documentation system + All Accessories & trays
  • Qsonica Q700 Sonicator; bath and probe available
  • Agilent 2100 Bioanalyzer
  • Typhoon Trio fluorescent gel scanner
  • Large capacity shaking incubator
  • Beckman Coulter ultra-centrifuges – Type 100ti, 45ti, and 50.2ti rotors available
  • L100XP (3rd floor B)
  • L90K (3rd floor A)
  • L100XP (1st floor A)