This facility is reserved for Biodesign faculty and staff only

Overview of Rules

• All Shared Instrument Facilities are free of charge to Biodesign faculty and staff
• All equipment is self-serve
• All instruments are used on a first-come, first-serve basis
• Access and Training for all instruments is coordinated and supported by Ian Shoemaker, ian.shoemaker@asu.edu
• Instrumental maintenance and calibration performed by Biodesign Facilities
• Lab courtesy rules apply
• ASU Gas Safety and instrument specific training required for Jasco J-815 Spectropolariometer
• Instrument specific training required for Nanosight NS300

Overview of Tools

Jasco J-815 Circular Dichroism Spectropolarimeter

Measures and quantifies differences in absorption of left and right circularly polarized light in chiral molecules and macromolecular structures

• Analyze secondary/tertiary structure or conformation of macromolecules
• Observe how secondary structure of proteins changes with environmental conditions or on interaction with other molecules
• Derive structural, kinetic and thermodynamic information
• ASU gas handling and instrument specific training required
• Researchers will provide their own cells / cuvettes; recommendations available based on application

Spectramax M5 plate reader (96/384 format)

Multimode photometrics for endpoint or kinetic measurements

• Absorbance (UV – VIS)
• Fluorescence
• Glow Luminescence
• Time Resolved Fluorescence
• Fluorescent polarization

ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX Calibrated

Fluorescence based monitoring of PCR in real time to quantify nucleic acid transcripts.

• Gene expression assays / Genotyping analysis
• High resolution melt analysis
• miRNA & siRNA quantification
• Single cell PCR
• TaqMan Array Card ready

Biorad C1000 Thermal Cycler

Two module tube strip (48 tubes per module) multi-use programmable thermal cycler

• Endpoint PCR
• Cloning reactions
• Provided as an overflow instrument for labs needing extra thermal cycler space
• Modules are independently programmable

GE ImageQuant LAS 4000

Basic CCD camera based blot / gel imaging 

• General Purpose gel and blot Imaging
• Chemiluminescence and fluorescence modes
• RGB Epi-illumination
• UV Trans-illumination
• Software allows incremental image accumulation
• NO ETHIDIUM BROMIDE GELS – “safe” stains only

NanoDrop 8000 Spectrophotometer

Simple, hand-pipetted pedestal based spectrophotometer

• 8 Channel model - 1uL required 2uL recommended
• Rapid DNA/RNA/Protein quantification
• Purity indicators A260/280 & A260/230 ratios
• Preconfigured methods for BCA, Bradford, Pierce 660, and Lowry assays

NanoSight NS300 Particle Characterization Platform

NTA (Brownian motion tracking) based size characterization and quantitation of particles

• 10 – 2000nm size range
• 10⁶ – 10⁹ particles / mL
• Viral vaccine and virus characterization
• Phage assembly
• Biomarker detection
• Protein aggregation
• Extracellular vesicle characterization

Hosted Equipment

Hosted items are owned or supported by facilities but located within individual biodesign centers that have a complimentary research focus and large user base.  All hosted items are available upon request and training completion, if applicable.  If you would like access to hosted equipment, to host a piece of equipment, or to offer an existing piece of equipment as a hosted item please contact Ian Shoemaker.

• BioRad GelDoc EZ gel documentation system + All Accessories & trays
• Qsonica Q700 Sonicator; bath and probe available
• Agilent 2100 Bioanalyzer
• Typhoon Trio fluorescent gel scanner
• Large capacity shaking incubator
• Beckman Coulter ultra-centrifuges – Type 100ti, 45ti, and 50.2ti rotors available
  • L100XP (3^rd floor B)
  • L90K (3^rd floor A)
  • L100XP (1^st floor A)