Protein Protein Interactions between the Viral and Host Proteome

Team: Xiaobo Yu, Ph.D., Ji Qiu, Ph.D., and Joshua LaBaer, MD, Ph.D.

Collaborators:  Kimberly Decker, Ph.D., M. Ramona Neuneubel, Ph.D. and Matthias P. Machner, Ph.D. (NICHD), Galan Jorge, Ph.D., DVM (Yale School of Medicine), and Sourav Ghosh, Ph.D. (Yale School of Medicine)



Proteins are the essential parts of all living cells and the functional change of proteins through protein-protein interactions can alter many cellular processes  such as protein transportation, gene transcription and signal transduction, etc. Furthermore, the proteins in host can be hijacked by the virulence factors of various bacterial pathogen/virus through physical binding to alter host immune response and facilitate the invasion/evasion and replication of their genome. Thus the study of how a protein interacts with upstream up-regulators and downstream effectors is at the core of understanding their molecular mechanisms in cells as well as in pathogen-host interactions. The results  would yield valuable information in the development of novel diagnosis and therapeutic treatment strategies for cancers and infectious diseases.

To address this question, we have developed a high-throughput unbiased protein-protein interaction screening platform on NAPPA microarrays displaying 10,000 human proteins and an independent validation platform on  magnetic beads using fluorescein labeled detection reagents with high-affinity and high-specificity. With these tools, we are performing a series of proteomics studies to characterize bacterial pathogen/virus and host interactions and identify the novel host targets which may play important roles in cancers and infectious diseases.