NAPPA Core Facility
Team: Jennifer Viloria and Michael Gaskin
In 2013, we established a protein microarray core facility. This core lab’s offerings are built around our unique NAPPA protein microarray platform. Using a patented method, full-length proteins are produced in situ on the array by human ribosomes and chaperone proteins minutes before testing them, ensuring that proteins stay naturally folded for the study. Our growing library of genes (available through DNASU) includes over 14,500 human proteins as well as clones that represent the entire genomes of several important human pathogens, over a dozen pathogenic viruses and some model organisms.
A key advantage of our method is that we can rapidly produce custom arrays of any proteins from our list of available genes and the robust expression method displays >95% of proteins, including large proteins (>100 kDa and membrane proteins). The amount of protein displayed per feature is approximately 100 fmol. Moreover, arrays can also be produced from other genes for which there are available cDNAs. We print and test thousands of arrays each year and careful quality control measures ensure CVs less than 20% between printings.
The offerings of our new protein array core include:
- Purification of plasmid DNA using our DNA Factory
- Creation of protein arrays
- Creation of protein arrays plus serum screening
- Creation of protein arrays plus serum screening and bioinformatics and statistical analysis of the results