Molecular Snapshots in the Initiation of Eukaryotic Transcription from Cryo-EM Studies

Molecular Snapshots in the Initiation of Eukaryotic Transcription from Cryo-EM Studies

September 23, 2016

Address

727 E. Tyler St.
Tempe, AZ 85287

Location

Biodesign Institute Auditorium

Date and Time

October 5, 2016, 3:00 pm (Length: 1 hour 0 minutes)

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Eva Nogales, Ph.D., Senior Faculty Scientist, Lawrence Berkeley National Laboratory

Initiation of transcription, a major molecular step is the regulation of gene expression, requires the assembly of general transcription factors into a large preinitiation complex that in humans form around the transcription start site. We are using single particle cryo-EM to obtain structural snapshots during the process of core promoter recognition, PIC assembly, DNA opening, loading of the transcribed strand into the active site of Pol II and initiation of mRNA synthesis. The use of direct detectors and advanced image analysis procedures has allowed us to significantly improve the resolution to gain detailed information on key protein-protein and protein-nucleic acid interactions during the complex process of eukaryotic transcription initiation.

This Biological Physics seminar is presented by the ASU Center for Biological Physics, the Biodesign Center for Applied Structural Discovery and the ASU Department of Physics.