Controversy surrounding red wolves and Mexican gray wolves clarified in new study

Controversy surrounding red wolves and Mexican gray wolves clarified in new study

April 2, 2019

  • The red wolf is a canine native to the southeastern United States. Once declared extinct in the wild, the red wolf has been reintroduced through captive breeding programs. Of 63 red wolves released from 1987–1994, the population rose to as many as 100–120 individuals in 2012, but has declined to 40 individuals in 2018 and today is severely endangered.


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  • Captive Mexican Wolf at Sevilleta National Wildlife Refuge, New Mexico. The Mexican gray wolf represents a smaller form of the gray wolf and inhabits a more arid ecosystem. 

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  • Michael Lynch, professor in the School of Life Sciences and director of the Biodesign Center for Mechanisms of Evolution at ASU, was one of the authors of a study that found red wolves are a distinct species and the Mexican gray wolf is a distinct subspecies.

    His research is focused on mechanisms of evolution at the gene, genomic, cellular, and phenotypic levels, with special attention being given to the roles of mutation, random genetic drift, and recombination.

    This work relies on the integration of theory development and computational analysis with empirical work on several model systems, including the microcrustacean Daphnia, the ciliate Paramecium, and numerous microbial species.


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April 2, 2019

Once, they roamed free in great numbers across the deserts, arboreal forests, grasslands and arctic tundra of the continental US. Today, wolf populations have been sadly depleted, the result of human ignorance, cruelty and loss of their vital habitats.

The red wolf and Mexican gray wolf are among the most endangered mammals in North America. Both species at one time were extinct in the wild. At last count, an estimated 114 wild Mexican gray wolves remain in the U.S. and only about 40 red wolves roam their native habitats in eastern North Carolina.

In recent years, efforts have been underway to help restore some of the nation’s wolf populations in the wild. Red wolves and Mexican gray wolves are listed as endangered under the U.S. Endangered Species Act, though their taxonomic status has been controversial and politically charged.

An expert panel, appointed by the National Academies of Sciences, Engineering and Medicine (NAS) has been conducting an analysis of scientific literature to answer the following two questions:

  1. Is the Red wolf a taxonomically valid species.
  2. Is the Mexican gray wolf a taxonomically valid subspecies.

The findings come at a critical moment for embattled wolves, as legislators, led by Republicans hostile to the natural world, seek to strip the gray wolf of protection under the Endangered Species Act. The identification of red wolves as a distinct species and Mexican gray wolves as an endangered subspecies may help to forestall government efforts to delist them. 

The study — undertaken by the Committee on Assessing the Taxonomic Status of the Red Wolf and the Mexican Gray Wolf — was sponsored by the U.S. Fish and Wildlife Service (USFWS). The National Academies are private, nonprofit institutions that provide independent, objective analysis and advice to the nation to solve complex problems and inform public policy decisions related to science, technology, and medicine. They operate under an 1863 congressional charter to the National Academy of Sciences.

Biological dilemma

Precisely defining a species can be challenging. Evolution is a continuous process and geographic variation among species is inevitable. These dynamic processes occur at different rates and follow distinct patterns for diverse taxa.

Nevertheless, when all the available biological data was examined, the prestigious panel of leading biologists agreed that red wolves (Canis rufus) are a distinct species and the Mexican gray wolf (Canis lupus baileyi) is a distinct subspecies. 

“The main issue is that although there are a number of definitions of species and subspecies, all lines of evidence in this case point in a coordinated way to the final conclusions,” says Michael Lynch, who directs the Biodesign Center for Mechanisms of Evolution at ASU. “Arriving at answers to the particular pair of questions evaluated was greatly facilitated by the unusual availability of whole-genome data previously obtained by researchers interested in the origin and diversification of North American canids, a relatively young component of the mammalian fauna of this continent.”

(Lynch’s research interests focus on the genetic mechanisms of evolution, particularly at the genomic and cellular levels, and on the development of methods for population-genomic analysis. His lab focuses on a number of model systems, most notably the microcrustacean Daphnia, the ciliate Paramecium, and numerous other unicellular prokaryotic and eukaryotic species.) 

In its new report released March 28th, NAS confirms the conclusion that both red wolves and Mexican gray wolves are indeed valid taxonomic identities.

According to the study's research guidelines: 

Determining the taxonomic status of the Mexican gray wolf and the red wolf requires an understanding of the contemporary meanings of species and subspeciesAll modern species concepts are united by the goal of identifying groups of organisms whose reproductive compatibility sustains genetic continuity.  Among the common principles that underlie multiple species concepts are (1) some level of reproductive isolation between different species that is mediated by genetic and ecological factors, and (2) phylogenetic continuity in time that is mediated by shared evolutionary history and inheritance. These principles provide a compelling, comprehensive approach to identifying species. Most modern concepts of subspecies rely on the notion of the partial restriction of gene flow, where subspecies are groups of actually or potentially interbreeding populations phylogenetically distinguishable from, but reproductively compatible with, other such groups.

Increasingly, genomic data reveal that gene flow among taxonomic groups through hybridization is a common feature of the evolutionary history of many widely accepted species, including wolves.

 

Mexican Gray Wolf

The Mexican gray wolf, whose range includes Arizona, New Mexico and northern Mexico was largely driven extinct from the wild in the U.S. in the mid-1970s. Then, 20 years ago the wolves were reintroduced, amid much controversy, into a small portion of their former range. Their designation as a subspecies has been controversial because there has been speculation that they are not physically or genetically distinct enough to justify that status. There has also been speculation that the current Mexican gray wolf population may have included ancestry from dogs or coyotes.

To determine whether the Mexican gray wolf is a valid subspecies, researchers evaluated the strength of the evidence for distinctiveness from other North American wolf populations, and the evidence for continuity between the historic Mexican gray wolf lineage and the contemporary population.

The report concludes that the Mexican gray wolf is a valid taxonomic subspecies of the gray wolf, based on the Mexican gray wolf’s size, morphology (physical characteristics such as head shape), and color, which distinguish it from other North American wolves. Genetic and genomic analyses confirm that the Mexican gray wolf is the most genetically distinct subspecies of gray wolf in North America.

The Mexican gray wolf represents a smaller form of the gray wolf and inhabits a more arid ecosystem than the gray wolf. Furthermore, the current managed population of Mexican gray wolves are direct descendants of the last remaining wild Mexican gray wolves; the known history of current Mexican gray wolves suggests that there is continuity between them and the historic lineage. There is no evidence that the genome of the Mexican gray wolf includes DNA from domestic dogs, the report adds.  

Red Wolf

The red wolf historically inhabited much of the eastern United States, but during the 20th century, populations were driven to very low numbers by habitat loss and predator eradication programs. Red wolves were largely replaced by coyotes that spread eastward from their original range in the western U.S. A few remaining specimens with red wolf morphology – physical characteristics long associated with red wolves – were captured in Texas and Louisiana before the red wolf went extinct in the wild and were used to establish a breeding program. 

The descendants of these animals were reintroduced in North Carolina and are now a managed population in the wild. The red wolf’s species status has been controversial because the wolves used to establish the breeding program were captured from a region where there had already been substantial interbreeding between red wolves, coyotes and gray wolves. 

Whether the red wolf is a valid species hinges on whether there is evidence that the historic population of red wolves has a distinct lineage, evidence that contemporary red wolf populations are distinct from gray wolves and coyotes, and evidence for continuity between the historic red wolf population and the contemporary one.

The evidence currently available supports the classification of the contemporary red wolf as a species distinct from gray wolves and coyotes, the report says. In addition, available evidence suggests that the historic red wolves constituted a valid species, and that contemporary red wolves trace some of their ancestry from these historic red wolves. However, genetic continuity between contemporary red wolves and the historic population cannot be firmly established without genomic data from ancient specimens.

Genomic DNA from historic specimens could help clarify this issue regarding continuity, the report says. In addition, more precise genetic analysis might help determine the exact proportion of the red wolf genome that has been replaced through historic interbreeding with coyotes and wolves.

“A majority of experts on red wolf taxonomy have concluded, time and time again, that the red wolf represents a unique lineage that is worthy of conservation and should remain a listable entity under the ESA,” according to Maggie Howell, Executive Director of the Wolf Conservation Center,  (one of many organizations devoted to wolf education and conservation). “No longer plagued by questions of taxonomy, USFWS needs to re-evaluate its recent decisions and management changes and bring its efforts back in line with the conservation mandate of the ESA." 

  • Michael Lynch is a member of the US National Academy of Sciences, and of the American Academy of Arts and Sciences; and is past president of the Genetics Society of America, the Society for the Study of Evolution, the Society for Molecular Biology and Evolution, and the American Genetics Association. Dr. Lynch was a member of the NRC Committee on Scientific Issues in the Endangered Species Act (1993 to 1995) and the Ecosystems Panel (1997 to 2000). Three widely cited books that he authored are Genetics and Analysis of Quantitative Traits (with Bruce Walsh, 1998), The Origins of Genome Complexity (2007), and Evolution and Selection of Quantitative Traits (with Bruce Walsh, 2018).

 

Written by: richard harth