Assistant Professor Research, Biodesign Virginia G. Piper Center for Personalized Diagnostics
Mitchell Magee joined the Virginia G Piper Center for Personalized Diagnostics as part of a team of scientists and engineers to assist with discovery of high throughput methods for analysis of protein-protein interaction and biomarker discovery. He attended the University of Texas Medical Branch and obtained a B.S. in Medical Technology. After several years’ experience in clinical and research laboratories, he matriculated to Texas A&M University and obtained a Ph.D. in Microbiology. His post-doctoral fellowship was performed at the University of Pittsburgh. He has been working extensively in interdisciplinary teams for high-throughput sample handling and analysis efforts. He helped initiate one of laboratories as part of the CDC’s National Tuberculosis Genotyping and Surveillance Network in which we performed RFLP typing of clinical isolates of Mycobacterium tuberculosis. Here we catalogued information on over 5,000 clinical tuberculosis isolates and established and validated DNA extraction and DNA fingerprinting analyses protocols. Additional experience with high throughput functional studies occurred in several projects of vaccine candidate discovery in models of coccidioidomycosis, smallpox, and glanders. These studies combined efforts of bioinformatics, molecular biologists and biologists to identify immunodominant proteins functionally through their protective effects against microbial challenge. He was also a key member of ASU’s efforts as part of the Tularemia Vaccine Development contract where he coordinated and performed gene expression microarray analyses of bacterial RNA harvested from the tissues of infected animals. Most recently, as part of the Biological Advanced Research and Development Authority contract to identify genes responsive to ionizing radiation, he instituted high throughput sample preparation standard operating procedures for preparing cDNA from blood for RNA-Seq and qPCR analyses. These large scale studies of gene expression, peptide and protein microarrays, provide a firm foundation for high throughput studies with an emphasis on developing and refining sample preparation and analysis protocols.