Ros Lab News
For this year's holiday event we tried to "escape the volcano island" and had our Christmas party at Dr. Ros’ house.
Austin Echelmeier graduated with a PhD from the Ros lab! His thesis title is Sample Delivery Enabled by 3D Printing for Reduced Sample Consumption and Mix-and-Inject Serial Crystallography at X-ray Free Electron Lasers
Towshif Rabbani receives the College Leaders Award. Congrats Towshif!
Soo many awards for Towshif this year... Good job!
Diandra Doppler, Mukul Sonker and Daihyun Kim presented a poster at the Nature Conference Functional dynamics – visualizing molecules in action, Nov 6-8 at ASU.
Diandra's poster was entitled 3D Printed Co-Flow Devices for Reduced Sample Consumption at the European XFEL and Daihyun's poster Droplet Triggering in 3D-Printed Microfluidic Devices for Serial Femtosecond Crystallography. Mukul presented on A Microfluidic Device for Rapid Screening of Protein Crystallization Conditions and Fixed-Target Applications.
Dai Hyun Kim presents at the µTAS conference in Basel, Switzerland
Dai Hyun presented two posters and receved a Biodesign Tavel Award as well as a BioXFEL travel grant to participate at the conference. Congrats Dai Hyun! The poster titles were Droplet Triggering in 3D-Printed Microfluidic Devices for Serial Femtosecond Crystallography and Sample consumption reduction for serial crystallography using water-in-oil droplets.
Towshif recevies 3 Awards for his poster at SciX in Palm Springs!
Twoshif presented his work on High-Resolution 3D-Printed Microfluidic Devices for Dielectrophoretic Applications at the SciX conference 2019 in Palm Springs. He received an AES poster Awards, a FACSS poster award AND a Biodesign Travel grant for his contribution. Congrats Towshif!
Other Ros lab members present at SciX as well:
Dai Hyun and Ana also presented posters at the SciX conference. Ana presented on both The role of gold nanoparticle size in the quenching-to-enhancement transiton of plasmon assisted upconversion luminescence and In line optical detector for aqueous-in-oil droplets in fused silica capillaries. Dai Hyun presented on Electrically triggered water-in-oil droplets for sample reduction in serial femtosecond crystallography. Mukul presented a poster and was selected for a talk during the AES annual meeting with his contribution entitled High-throughput Deterministic Ratchet Devices for Organelle Separation.
Our work on droplet generation for SFX published in the Journal of Applied Crystallography:
Echelmeier et al., 3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating, Journal of Applied Crystallography (2019) 52, 997-1008, DOI:10.1107/S1600576719010343
Our review Article by Echelmeier, Sonker, Ros entitled Microfluidic sample delivery for serial crystallography using XFELs goes online!
Full citation: A. Echelmeier, M. Sonker, A. Ros Microfluidic sample delivery for serial crystallography using X-ray free electron lasers, Analytical and Bioanalytical Chemistry (2019) 441, 6535–6547 DOI:10.1007/s0021
Towshif receive the John Kacoyannakis Award!
Every year, the School of Molecular Sciences awards the John Kacoyannakis Award to an outstanding PhD student in Analytical Chemistry. Towshif Rabbani was this year's recipient. Congrats Towshif!
Daihyun presents a poster at MSB 2019 an is first runner-up in the poster competition!
Congrats Daihyun on your 2. place poster award! His poster title was A Microfluidic Ratchet for Sub-micrometer (Bio)Particle Separation
Four presentations at the Biodesign Fusion Event from the Ros lab!
Towshif, Jorvani, Dai Hyun and Austin presented posters and Austin received the "Einstein Award" for his poster on 3D-Printed Microfluidic Devices for Mix-and-Inject Serial Crystallography at X-ray Free Electron Lasers. Congrats Austin!
Four Ros Group students participate at the 2019 Biophest:
Towshif with poster Continuous fractionation of single-walled carbon nanotube with insulator based dielectrophoresis
Jorvani with poster Alzheimer's Disease Protein Analysis from Microdissected Brain Cells with Microfluidics MALDI Mass Spectrometry
Dai Hyun with poster Sub-micrometer (bio)particle separation using a deterministic ratchet microfluidics
Austin with poster Mix-and-Inject Serial Femtosecond Crystallography Facilitated by 3D-Printed Microfluidics
Our work on time-resolved crystallography of cytochrome c oxidase in collaboration with the Rousseau group published in PNAS
Daihyun Kim and Sahir Ghandi presented a poster at the 6th Annual BioXFEL International Conference in San Diego and contributed to the Workshop on Sample Delivery.
Daihyun's poster was on Revolution in Sample Reduction for SFX: Droplet-based Sample Delivery and Triggering. Austin's poster was on Time-Resolved Mix and Inject Serial Crystallography Facilitated by 3D-Printed Microfluidics. He won a poster award at the conference for this contribution! Congrats Austin!
Jorvani at AZ Bio 2019:
Jorvani presented a poster at the AZ Bio 2019 event in Phoenix (Alzheimer's Disease Protein Analysis from Microdissected Brain Cells using Microfluidics and MALDI Mass Spectrometry).
Ros Lab PhD students participate at the SMS visitation weekend
Poster presenters were Towshif, Jorvani Dai Hyun, Austin. They presented on their PhD projects.
We enjoyed bowling and had our group Christmas party at Dr. Ros’ house.
Towshif’s experiment on continuous fractionation of Single Walled Carbon Nanotubes (SWNTs) by length with iDEP continues at Georg-August-Universität Göttingen, Germany
Towshif went to Georg-August-Universität Göttingen, Germany, for a collaborative project with Dr. Christoph Schmidt where he is working on the purification and fractionation of SWNT with iDEP techniques. Previously, he published the first study of iDEP characteristics of SWNT in the low frequency range. Now, he is focusing on fractionation of SWNT by length with an iDEP-based microfluidics sorter device based on his previous iDEP study.
New Advanced Photolithography Lab in Biodesign C, CASD – Ros Lab
Class 1000 level clean room and a mask aligner (Karl SUSS MJB4) for the microfabrication and photolithography are up and running.
Ros group published an invited review article in Analytical Chemistry.
Daihyun Kim, Dr. Mukul Sonker and Dr. Alexandra Ros published a review in Analytical Chemistry. The title of the work is “Dielectrophoresis: From Molecular to Micrometer-Scale Analytes”. This review discusses the fundamental theory of dielectrophoresis (DEP) as well as methods and applications for analytes in a size range as small as biomolecules such as proteins or nucleic acids over organelles, various microbes up to blood cells. Accepted Nov 27, 2018. DOI: 10.1021/acs.analchem.8b05454
MFX beam time at LCLS, Stanford
Our team members, Austin and Sahir, participated in a beam time taking place at LCLS (at SLAC National Accelerator Laboratory) to test 3D-printed microfluidic devices to quickly mix oxygen into an anaerobic cytochrome c oxidase crystal suspension, and subsequently inject the mixture into an X-ray free electron laser for time-resolved serial crystallography experiments with other collaborators from Albert Einstein College of Medicine, NY, including Dr. Izumi Ishigami, Dr. Syun-Ru Yeh, and Dr. Denis Rousseau.
Dr. Alexandra Ros received the FACSS 2019 Innovation Award
Dr. Alexandra Ros received an innovation award from the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) for her paper “Electrically Triggered Water-in-Oil Droplets for Serial Femtosecond Crystallography” and presentation at SCIX 2018, Atlanta, GA. Congratulations!
Daihyun and Towshif presented their work at SCIX 2017
Daihyun presented his work on “A deterministic ratchet for sub-(bio)micrometer particle separation” and “A Versatile Droplet Generator using Electric Triggering for Serial Femtosecond Crystallography Application” with two posters at SciX 2018. Towshif present his work on “Continuous Fractionation of Single-Walled Carbon Nanotubes by Length with Insulator-based Dielectrophoresis” at the same conference within the AES poster session. Daihyun received travel grants from NSF and GPSA, and Towshif also received NSF travel grant for the participation in the SciX conference. Congratulations!
Two new undergrads joined the group
Manuel Guzman (left) and Alonso Aceves (right) joined the group to be involved in various research projects in the Dr. Ros Lab. A warm welcome to Manuel and Alonso!
NIH supplement proposal funded.
Dr. Fromme and Dr. Ros recently submitted a supplement proposal for the development of a Fixed Target Microfluidic Device and Stage for crystallographic studies at the new home source Compact X-ray Free Electron Laser (CXFEL), currently being developed at ASU. Supplement proposal was funded through NIH in September.
2018 LCLS/SSRL User Meeting
Austin presented a poster on his work with microfluidic methods for time-resolved serial crystallography at the 2018 LCLS/SSRL User Meeting.
Biodesign Institute Building C Grand Opening
Daihyun, Jorvani, Towshif, Mukul and Sahir volunteered for the Biodesign C Grand Opening event where 450 researchers, faculty, staff, students, city and state officials, as well as members of the public gathered to celebrate the opening of Biodesign C.
Diandra joined the group
Diandra Doppler joined the group for the summer BioXFEL internship program. She will be working on 3D-printed Nozzles, droplet generation and a droplet triggering device in her project. A warm welcome to Diandra! (She continued as an undergraduate researcher in the fall of 2018 and spring of 2019 in the Ros lab.)
Dr. Ana Egatz-Gomez published in Physical Chemistry C
Dr. Ana Egatz-Gomez participated in a project at University Complutense of Madrid, Spain, with Dr. Sonia Melle, Oscar G. Calderón, Marco Laurenti, Diego Mendez-Gonzalez, Enrique López-Cabarcos, E. Cabrera-Granado, Elena Díaz, and Jorge Rubio-Retama. This work has been published with the title: “Förster Resonance Energy Transfer Distance Dependence from Upconverting Nanoparticles to Quantum Dots” in the ACS Journal of Physical Chemistry C. This work discusses nanoparticle systems with alternative architectures for efficient Förster resonant energy transfer. It has been sponsored under ACS Editors Choice for immediate, free open access by ACS due to its potential for broad public interest, an honor given to only one article from the entire ACS portfolio each day of the year. DOI: 10.1021/acs.jpcc.8b04908
Ros Lab moved into Biodesign C.
Ros Laboratory settled down to its new home at the multi-functional research-oriented Biodesign C building as a member of the Center for Applied Structural Discovery.
Sahir, Mukul, Daihyun, and Austin presented posters at Biophest 2018, Tucson
Dr. Sahir Gandhi, Daihyun Kim and Austin Echelmeier presented a poster on “Actively reducing protein crystal consumption by electrically triggering droplets for Serial Femtosecond Crystallography”. Dr. Mukul Sonker presented a poster on “COC-PDMS Microfluidic Devices for in-Lipidic Cubic Phase (LCP) Protein Crystallization” at Biophest 2018 held on University of Arizona's Thomas W. Keating Bioresearch Building.
Prof. Ros recognized for 10 years of service at ASU
Dr. Ros received her 10 year's of service award from the School of Molecular Science together with department collegues at the Annual Service Award get together.
Jorvani Cruz received CLAS Graduate Student Excellence Award.
Jorvani was awarded the Excellent Student Award from the College of Liberal Arts and Sciences at ASU. Congratulation!
Daihyun received SMS Research Assistant Award
Daihyun was awarded a Research Assistant Award at Arizona State University's School of Molecular Sciences in recognition of his outstanding graduate reserach. Congratulation!
Ros group and BioXFEL team returned for a beam time at EuXFEL, Germany.
Dr. Ros, Ana, Austin and Daihyun and the BioXFEL team participated at another EuXFEL beam time. Tunable droplet generation, based on electrical triggering, was successfully tested at the European X-ray Free Electron Laser (EuXFEL) facility in Hamburg, Germany.
NIH funds Ros Lab Research.
NIH awarded an R01 grant to Dr. Ros for developing new fractionation methods for organelles by type and size. NIH funds this reserach through a Research Project Grant (R01), which is the original and historically oldest grant from NIH. The funds will be used to develop efficient organelle serparation mechanisms and design microfluidic devices to fractionate organelle subpopulations to enable breakthrough biomedical research.
The Night of the Open Door at ASU
Towshif Rabbani participated in this positive and enthusiastic event held at the Biodesign center. We showed our protein crystallization device and surface coated substrates in the event. https://opendoor.asu.edu/tempe
Daihyun published in Analytical Chemistry.
Daihyun’s work has recently published in Analytical Chemistry. He developed a ratchet-based microfluidic device capable of fractionating biological particles. This method is a promising separation technique for sub-micron size of organelles.
We explored the South Mountain park and had our group Christmas party.
Dr. Sahir Ghandi joined the group as Post Doc to support our research projects. A warm welcome to Sahir!
Sebastian Quintana joined the group for undergraduate reserach. A warm welcome to Sebastian!
Towshif published in Analytical Chemistry.
Towshif’s work has recently published in Analytical Chemistry where he reported the first study of iDEP characteristics of single walled carbon nanotubes at low frequencies. This study is fundamental for further utilizing iDEP in separation and purification applications for SWNTs.
Dr. Mukul Sonker joined the group as Postdoctoral Researcher. A warm welcome to Mukul!
Anikki Giessler presented her work at AES 2017
Anikki Giessler presented her work on clustering behavior of DNA under AC dielectrophoresis conditions and won a poster award at the 2017 American Electrophoresis Society (AES) annual meeting. Congratulation!
Daihyun Kim presented his work at SCIX 2017
Daihyun presented his work on adeterministic ratchet for sub-micrometer particle separation and won a poster award at the 2017 SCIX conference AES poster session. Congratulation!
Dr. Ros group among the first user groups at Europe’s brightest light source
AN ASU team lead by Dr. Alexandra Ros has selected by an international committee of experts for the first user experiments at newly opened European X-ray free electron laser facility (EuXFEL) in Hamburg, Germany. Our group members, Austin Echelmeier, Jorvani Cruz Villarreal and Ana Egatz-Gomez participated together with other reserachers from ASU and the EuXFEL to establish a novel microfluidic system improving sample delivery of protein crystals to the EuXFEL.
MFX beam time at SLAC, Stanford
Our team participated in a beam time taking place at the SLAC LCLS facility to test a droplet-based system for sample consumption reduction on XFEL crystallography experiments.
Fabiola M Moreno Echevarria joined the group for the summer intern program. A warm welcome to Fabiola!
Biodesign Fusion 2017 - Scientific Retreat
The Ros group attended the Biodesign Fusion 2017 event (scientific retreat and poster session) in Carefree Resort. Austin Echelmeier gave a 2 min pitch talk on Microfluidic Mixing Methods for Serial Crystallography. Daihyun Kim, Mohammad Towshif Rabbani, Austin Echelmeier and Ana Egatz presented their work in the poster session.
Daihyun Kim receives CASSS travel grant for MSB2017
Daihyun Kim’s work on Organelle fractionation for subpopulation analysis and Dynamic Constriction Insulator-Based Dielectrophoresis for Particle Manipulation was presented as an oral presentation and a poster at MSB (Microscale Separation Bioanalysis) 2017 conference in the Netherlands.
The Night of the Open Door at ASU
All lab members of the Ros group participated in this positive and enthusiastic event held at the Biodesign center. https://opendoor.asu.edu/tempe
Paul Jones presents at SciX 2017
Paul Jones presented at SciX 2017 with a talk entitled Dielectrophoretic Response of Condensed DNA Clusters in AC fields.
Jorvani Cruz Villarreal joined the group as a Ph.D student. A warm welcome to Jorvani!
Paul Jones and Gabe publish in Analytical Chemistry
Paul Jones and Gabe’s work was recently published in Analytical Chemistry. The title of their work is Continuous Separation of DNA Molecules by Size Using Insulator-Based Dielectrophoresis.
Accepted Dec 12,2016. doi: 10.1021/acs.analchem.6b03369
Group Hike in the South Mountains
Our Christmas group hike was in the South Mountains in Phoenix.
Ros Lab Participates at the Biodesign Mixer Event
Austin Echelmeier, Paul Jones, and Daihyun Kim presented various examples of our work in the 2016 Biodesign Mixer.
Mian Yang publishes in Analytical Chemistry
Mian Yang’s work was recently published in Analytical Chemistry. The title of the paper is Towards Analysis of Proteins in Single Cells: A Quantitative Approach employing iTRAQ labels with MALDI Mass Spectrometry Realized with a Microfluidic Platform.
Accepted Jun 3, 2016. DOI: 10.1021/acs.analchem.5b03419
Jinghui Luo publishes in Analytical Chemistry
Jinghui Luo’s work was recently published in Analytical Chemistry. The title of the paper is Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device.
Accepted May 5, 2016. DOI: 10.1021/acs.analchem.6b00837
Bahige Abdallah and Mian Yang graduate
Bahige Abdallah Abdallah graduated in the Spring of 2016 with his thesis entitled Microfluidic Tools for Protein Crystallography. Mian Yang Yang graduated in the Spring of 2016 with her thesis entitled Hyphenation of a Microfluidic Platform with MALDI-TOF Mass Spectrometry. Congratulations!
Bahige Abdallah publishes in the journal Crystal Growth and Design.
Bahige Abdallah’s work was recently published in Crystal Growth and Design. The title of the paper is Protein Crystallization in an Actuated Microfluidic Nanowell Device.
Accepted Feb 25, 2016. DOI: 10.1021/acs.cgd.5b01748
Fernanda Camacho Publishes in Bioanalysis
Fernanda Camacho’s work was recently published in Bioanalysis. The title for her work is Protein Dielectrophoresis and the Lin Gank to Dielectric Properties.
Accepted Feb 7, 2016. DOI: 10.1039/c4an01503g
Lin Gan Publishes in Analytical Chemistry
Lin Gan’s work was recently published in Analytical Chemistry. The title of her work is Polarizability of six-helix bundle and triangle DNA origami and their escape characteristics from a dielectrophoretic trap.
Accepted Nov 16, 2015. DOI: 10.1021/acs.analchem.5b02524
Bahige Abdallah publishes in the journal Structural Dynamics and Analytical Chemistry
Bahige Abdallah’s work Microfluidic sorting of protein nanocrystals by size for XFEL diffraction was recently published in Structural Dynamics. He also published an article in Analytical Chemistry entited Protein Nanocrystal Sorting Optimized with Numerical Simulation.
Accepted Aug 19, 2015. DOI: 10.1063/1.4928688
Austin Echelmeier Publishes in the 19th µTAS Proceedings
Austin Echelmeier’s work was recently published in 19th µTAS Proceedings. The title of his work is Biphasic Droplet-Based Sample Delivery of Protein Crystals for Serial Femtosecond Crystallography with an X-Ray Free Electron Laser. www.rsc.org/images/LOC/2015/PDFs/Papers/1374_M.361e.pdf
Ros BioXFEL team participates at June beam time at SLAC
Austin Echelmeier's presents his work resulting from June beam time at µTAS 2015!
Dr. Ros Awarded an Alexander-von-Humboldt Fellowship
Dr. Ros will be performing research at Georg-August-University Goettingen (Germany) in 2015-16 and was awarded a prestigeous award from the German Alexander-von-Humboldt-Foundation.
Bahige Abdallah comes home from SciX 2015 with two poster awards
Bahige Abdallah's poster 'Microfluidic methods to grow and fractionate membrane protein crystals' was awarded an AES and a FACSS poster award at SciX 2015 conference!
Ros BioXFEL Team awarded PCS Beamtime at SLAC
Read the resulting paper 'XFEL diffraction from protein nanocrystals isolated using a microfluidic sorter' at http://scitation.aip.org/content/aca/journal/sdy/2/4/10.1063/1.4928688.
Bahige Abdallah wins the John Kacoyannakis (Koko) Award
Spring 2014:Another student from the Ros lab was selected for the John Kacoyannakis (Koko) Award . CongratulationsBahige Abdallah! Bahige Abdallah was selected for his outstanding research, his excellent teaching and his engagement in outreach activities.
Jinghui Luo receives HPLC Inc. travel grant to HPLC 2014...
Congratulations to our graduate student Jinghui Luo Luo who received the HPLC Inc. travel grant for 2014. Jinghui Luo will present her work on insulator-based dielectrophoresis of mitochondria at HPLC 2014 in New Orleans in a talk.
Asuka Nakano presents at Pittcon 2014...
Asuka Nakano was our lab's delegate at Pittcon 2014. She presented a poster on 'Protein Dielectrophoresis using Insulator-Based Devices: Implications at Nanoconstrictions'. Asuka Nakano was supported by the CLAS travel grant for presenting at Pittcon 2014.
Ros Lab Christmas Hike
We explored the South Mountain Park in Phoenix in a ~4h hike including hidden valley...
Sanchari Bhattacharya defends her thesis...
Sanchari Bhattacharya Bhattachary graduated in December 2013. She defended her thesis entitled 'Insulator Based Dielectrophoretic Trapping of Single Mammalian Cells'. Congratulations Sanchari Bhattacharya! Sanchari Bhattacharya now works at Marlyn in the Phoenix area.
Bahige Abdallah publishes in ACS Nano... Story #1
Bahige Abdallah Abdallah's work was recently published in ACS Nano! He developed a microfluidic sorter capable of fractionating membrane protein nanocrystals such as photosystem I crystals into nm-sized fractions. This method is a promising sample preparation method for nanocrystallography. Read more in his article...
Bahige Abdallah publishes in ACS Nano... Story #2
Bahige Abdallah Abdallah's also published his work on protein cyrstallization in ACS Nano! He developed a microfluidic platform tostudy crystallization of membrane proteins to construct phase diagrams and reveal the conditions for nanocrystal growth. Read more in his article...
Jinghui Luo wins 2 poster prizes at SciX
Jinghui Luo presented her work on dielectrophoresis of mitochondria at SciX and impressed the award committees with her work. She won both an AES student poster award and a FACSS student award. Congratulations Jinghui Luo!
Photo (from left to right): Dr. Fernanda Camacho Camacho (Chair of Judging Committee, Jinghui Luo Luo (Poster Award Winner), Dr. Alexandra Ros (Jinghui Luo's advisor)
Asuka Nakano wins the John Kacoyannakis (Koko) Award
Spring 2013: Asuka Nakano wins the John Kacoyannakis (Koko) Award, the department's award presented to an outstanding graduate student. Asuka Nakano was awarded for her pioneering work on protein dielectrophoresis and her productivity during her PhD. She is planning to graduate in Spring 2014. Congratulations Asuka Nakano!