T-cell responses in tularemia

Investigators:  TVS Murthy, Ph.D. (former HIP member), Marcin Pacek, Ph.D. (former HIP member), Andreas Rolfs, Ph.D., Zhenwei Shi (Research Assistant)

Collaborator: Dennis Kasper and Lee-Ann Blalock; Department of Microbiology and Molecular Genetics (HMS)

Together with the Kasper group, an assay was developed to use purified proteins as antigen source for ELISPOT assays. We have used our complete F. tularensis clone collection to produce proteins in E.coli in high throughput for a genome wide screen for proteins that induce a T-cell response. Potential hits have been identified and are currently tested in animal vaccine studies.