Innate immunity in tularemia

Investigators: Andreas Rolfs, Ph.D., Zhenwei Shi (Research Assistant)

Collaborator: Prof. D.L. Kasper, Department of Microbiology and Molecular Genetics (HMS)

Innate immunity studies were initiated for the human pathogen F. tularensis to further understand the specific immune biology of this bacterium. Using the ORF clone collection for F. tularensis previously published (Murthy, et al.) we expressed the entire proteome for this organism in vitro after transfer into an appropriate vector, and used these proteins to stimulate a toll-like receptor (TLR) response in immunological active cells. For further characterization, proteins were chosen that exhibited in independent experiments stimulation similar to that of a known TLR agonist.

A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis. Murthy T, Rolfs A, Hu Y, Shi Z, Raphael J, Moreira D, Kelley F, McCarron S, Jepson D, Taycher E, Zuo D, Mohr SE, Fernandez M, Brizuela L, Labaer J. (2007)